You are confusing the formats: FASTA + FASTA quality (http://en.wikipedia.org/wiki/FASTA_format) versus FASTQ (http://en.wikipedia.org/wiki/FASTQ_format ) As the story unravels and the puzzle comes together from the bits and pieces of your mails, it seems that you tried all the time to load a FASTQ file as FASTA quality file. This obviously cannot work. Now, please follow step by step the advice Lionel just gave you in his last mail and things should be running smoothly. Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html