Dear Sharmista, 1: do you have all the necessary files in the folder you are running mira? 2: do they have all the correct names? please provide a list of the files 3: I think The command should be: mira -project=my_project -job=mapping,genome,accurate,solexa -AS:nop=1 -SB:lsd=yes:bsn=myproject_ref:bft=gbf:bbq=30 Ciao D. ----- Messaggio Originale ----- Da: Sharmista Saha <sharmistasaha@xxxxxxxxxxxxxx> Data: Mercoledi', Settembre 30, 2009 8:58 am Oggetto: [mira_talk] Re: Assembly A: mira_talk@xxxxxxxxxxxxx > The command I typed was as follows: > sharmistha@PLEX- > 1:~/Sharmistha/Bioinfo/Project_data/my_project/mira/Dataform>mira > --project=my_project --job=mapping,genome,accurate,solexa* > *-AS:nop=1 > > * -SB:lsd=yes:bsn=myproject_ref:bft=gbf:bbq=30 > >&log_assembly.txt } > > > > *This was my comand line.......... > > now can you suggest me, where am I wrong? > > Thanks and Regards, > > Sharmistha > > > > On Wed, Sep 30, 2009 at 12:14 PM, Sven Klages > <sir.svencelot@xxxxxxxxxxxxxx>wrote: > > > You still didn't provide the command you typed in. So it's > difficult to > > tell what went wrong. > > > > cheers, > > Sven > > > > 2009/9/30 Sharmista Saha <sharmistasaha@xxxxxxxxxxxxxx> > > > > Can anyone solve my required issue? I need it very much and am > very curious > >> to see how my denovo assembly happens via mira,. > Please, if anyone can > >> direct me my error in execution of mira, I shall be highly > grateful. I am > >> still positive about the tool to work in my sequence too, > when it can > >> resolve so many queer issues. > >> > >> Only my sequence is a solexa sequence. waiting > hopefully for some > >> positive response on the same ground. > >> > >> Thanks and Regards, > >> Sharmistha > >> > >> > >> On Fri, Sep 25, 2009 at 2:06 AM, Bastien Chevreux > <bach@xxxxxxxxxxxx>wrote:>> > >>> On Donnerstag 24 September 2009 Sharmista Saha wrote: > >>> > [...] > >>> > This is what I got. Hope this answers your query. > >>> > >>> Not entirely, you forgot the command line you typed in. > Fortunately, the > >>> directory listing you provided gives a hint to what happened. > >>> > >>> Do NOT type the command line prompt that is shown in the > examples, it's > >>> NOT > >>> part of the mira command! > >>> > >>> Hope that helps. > >>> > >>> Regards, > >>> Bastien > >>> > >>> > >>> -- > >>> You have received this mail because you are subscribed to > the mira_talk > >>> mailing list. For information on how to subscribe or > unsubscribe, please > >>> visit http://www.chevreux.org/mira_mailinglists.html > >>> > >> > >> > > >