[pure-silver] Re: Pyro in a Jobo Processor
- From: "Breukel, C. (HKG)" <C.Breukel@xxxxxxx>
- To: <pure-silver@xxxxxxxxxxxxx>
- Date: Mon, 21 Mar 2005 10:18:38 +0100
> -----Original Message-----
> From: pure-silver-bounce@xxxxxxxxxxxxx
> [mailto:pure-silver-bounce@xxxxxxxxxxxxx]On Behalf Of John Black
> Sent: vrijdag 18 maart 2005 19:51
> To: pure-silver@xxxxxxxxxxxxx
> Subject: [pure-silver] Re: Pyro in a Jobo Processor
>=20
>=20
>=20
> The tris/borate dry packaged buffers I get from sigma already=20
> have the EDTA
> in them. These buffers are intended for use in electrophoresis and the
> reason for the EDTA in these circumstances escapes me.
>=20
..Hi Jim,
I also use these buffers regularly (actually right now), and I always
understood that the EDTA was essential for the conductivity(?) during
electrophorsis, but how this exactly works I cannot remember..., but
omitting EDTA gives terrible running behaviour of DNA molecules..ok
enough off-topic..;-)..
Best,
Cor
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