Not sure if this is helpful, but I made a recent 3.5 million 454 assembly (~350 bp ave length) that took 10 days. This was a transcriptome assembly at accurate setting. Our system also has 512 GB memory, 4 hexacore processors (I believe). Number of processors doesn't seem to affect the assembly too much, as I believe it is only parrallelized during the skimming process. NFS is a bad idea; my first run stalled and had to be killed. Is your run log file 500 GB or the log directory in the assembly folder? Michael On Tue, Dec 21, 2010 at 3:09 AM, Ganga Jeena <ganga.jeena@xxxxxxxxx> wrote: > I have to do this assembly for 8 million reads454 in master-node/home > within 230 GB space. How should I work it? > The log file generated is already 500 GB. > Is there some way | option to avoid log file generation and other > non-result files ? > Regards, > Ganga Jeena > > > > On Tue, Dec 21, 2010 at 10:22 AM, Ganga Jeena <ganga.jeena@xxxxxxxxx>wrote: > > >> >> ---------- Forwarded message ---------- >> From: Bastien Chevreux <bach@xxxxxxxxxxxx> >> Date: Mon, Dec 20, 2010 at 11:54 PM >> Subject: [mira_talk] Re: Regarding Time-estimate of mira >> To: mira_talk@xxxxxxxxxxxxx >> >> >> On Montag 20 Dezember 2010 Ganga Jeena wrote: >> > I am trying to do assembly of 8 million reads with mira >> > Configuration of system is as follows: >> > 512 GB RAM >> > 8 hexa-core processors >> > >> > miramem gives me a memory estimate of 66.6 GB. >> > How to get the time-estimate for assembly of data on my system. >> >> >> It is impossible to estimate the time needed, there are too many >> sequencing >> project depending variables which make things absolutely unpredictable. >> >> Sorry, you'll have to try it out with your data. >> >> B. >> >> -- >> You have received this mail because you are subscribed to the mira_talk >> mailing list. For information on how to subscribe or unsubscribe, please >> visit http://www.chevreux.org/mira_mailinglists.html >> >> >