> Why program yourself if it's already present? > > Adnan: if you used strain information for your reads, try > > convert_project -f (maf or caf) -t fasta ... > > which will output different FASTAs for each strain present in the assembly. > Then grep the FASTAs for the '@' sign ... these are the places where no read > from a given strain covers the place. Yes, i will try it, but i am afraid as I read that convert project will list the desired coverage contigs in multifasta format and it will not insert N's between the contigs. For example: Zero coverage region b/w two contigs is 200bp long, so there should be 200 N's b/w contigs in fasta file. But i will have to check it before saying anything. Regards /adnan _________________________________________________________________ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2