Hi all, Can anyone tell me is it possible to get scaffolded contigs in MIRA mapping. I mean after mapping reads with reference sequence, some regions little or no coverage. But when i view both padded and unpadded consensus fasta file, the contig before zero coverage region and contig after zero coverage is joined with reference sequence. I dont want bp of reference sequence to be added in the fasta file. Is there some way i can insert NNNNN or ***** between those contigs? Hope i cleared my problem. Regards /adnan _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4