[mira_talk]
- From: Mehmet göktay <mehmetgoktay1989@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 25 Jul 2014 13:43:51 +0300
Hello everybody,
I'm trying to assembl artificial reads which produced by art which is a
next generation sequencing simulator. I do have illumina reads in
different coverages. The problem even though I used e.coli genome to
produce reads in 10x, 20x, and even 30x coverages I got dozens of contigs
and the lenght of the contigs very short. (I meant it because lots of
contigs length same with the read lengths (100bp)).After 40x coverage I do
get better contig lengths but still the number of contigs so high!!! at 40x
coverage I end up with 4000 contigs.
I used very basic parameters to run mira. Now I'm reading manuals of it but
I almost lost in it. Is there anybody can give me advice that can improve
my result?
--
Mehmet Göktay, MSc student
Department of Molecular Biology and Genetics
Izmir Institute of Technology
35430, Urla, Izmir, TURKEY
(For the website of Plant Molecular Genetics Laboratory please click here
<http://plantmolgen.iyte.edu.tr/>.)
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