Dear sir Successfully the reads (DRA00584, DRA00585)were assembled into a total 14,132 & 13,490 contigs respectively (without preprocessing) using MIRA . For the same strain Paracel TranscriptAssembler version 2.6 software gave a 27.427 & 29,036 non redundant sequence respectively , were acquired Ref( Bioresource Technoogy Watanabe.. et all 2012 ) . I need to know why there is such a wide variation in the count. Does MIRA require the pre processing step before assembly? -- *Thanks & Regards,* * G.Vishnupriya *