The actual command I am using is: mira --projectin=LRP2_mm --projectout=LRP2_mm_I12 --job=denovo,est,accurate,454,solexa 454_SETTINGS -CL:emlc=yes -CL:mlcr=16 -CL:emrc=yes -CL:mrcr=20 COMMON_SETTINGS -GE:mps=12 This is transcriptome data, so I was using the "est" job setting. -Marshall On Tue, Apr 19, 2011 at 8:11 AM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote: > On Apr 19, 2011, at 14:35 , Marshall Hampton wrote: >> I am getting the following error on a lot of little assemblies I am >> doing right now. They are 454 and solexa combinations. The solexa >> data might have been preprocessed in a suboptimal way. >> >> My input can be found at: >> http://www.d.umn.edu/~mhampton/LRP2.tar.gz >> I used mira 3.2.1.15. > > Ummm ... strange. I tested with your input like this: > > mira --project=LRP2_mm -job=denovo,genome,accurate,solexa,454 > > and the assembly went through without any problem. > > What command line are you using? > > B. > > > -- > You have received this mail because you are subscribed to the mira_talk > mailing list. For information on how to subscribe or unsubscribe, please > visit http://www.chevreux.org/mira_mailinglists.html > -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html