[mira_talk] Re: Vector screen
- From: Sven Klages <sir.svencelot@xxxxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 15 Mar 2011 11:52:48 +0100
2011/3/15 Leonor Palmeira <mlpalmeira@xxxxxxxxx>
> [...] Well, from my own point of view, for a whole BAC 454 sequencing, I
> prefer to assemble the sequence containing the BAC as a whole, and then
> excise the BAC from the assembly. This allows me to make sure the assembly
> ran well, including around the BAC. I don't see the necessity for vector
> clipping the reads in this particular case.
>
This may work if you have one library per BAC. But in my case, we have a
pool of fosmids (or bacs or whatever clone constructs).
Assembling these without prior vector removal might lead to strange results.
Sven
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