On Monday 14 March 2011 21:15:13 Sven Klages wrote: > What part of the pre-processing software is capable of vector clipping? > You can easily clip 5' adaptors, maybe some basic 3' adaptor clipping, but > that's it. > There is no vector clipping 'module' in the pre-processing pipeline. Oops, hold on a minute. What I meant is: I never really saw 454 SFF files (those created by the Roche software) which had, when done "correctly"[1], adaptors left in the unclipped data. Now the quesion I was asking myself is why anyone would want to do a "vector" trimming on that data. Just wondering here ... > The assembler itself has options to do vector screening and trimming prior > to the assembly process. But this is not usable outside the assembler > suite. Wasn't that just something added to Newbler to be able to mix 454 and Sanger (the latter could have vectors)? B. [1] whatever "correctly" is ... I just saw the SFFs as delivered by sequencing providers and they were "good" -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html