Le 18/09/2012 10:25, Peter Cock a écrit :
On Tue, Sep 18, 2012 at 9:18 AM, Laurent MANCHON <lmanchon@xxxxxxxxxxxxxx> wrote:something wrong, after adding /1 and /2 at the end of headers into the respective files i have the same error: "Read ...... is present more than once in the data set. Did you load a file twice in the manifest? Is a read present more than once in your file(s)?" head GAMMA-1.solexa.fastq @D61655M1:276:D10YJACXX:8:1101:1456:1955 /1 ...Add the /1 and /2 without a space in front of them. Peter
nothing change, i have the same error message. head GAMMA-1.solexa.fastq /1@D61655M1:276:D10YJACXX:8:1101:1456:1955 NCTGNAGTGTNNGATNCGGGGTTNNNNNNNNNNACNTTNNNNNNNNNNGNNNGNNNTTGAANNNNNNNNTNACNGTNTNATNAANNNNTTCGGACNACAT + #0;@#2@=@?##32@#2=>@@<@############################################################################# /1@D61655M1:276:D10YJACXX:8:1101:1494:1967 NTTTNGGCTCTGTACCTTTTGTATCAGGGGAACCTAAAAGTGTAAAAAGTATACAATCGAGTGGGTTAGATCTTTCTAAGACTAGTTTAAAGTTAAATTG + #0;@#28=????9@?<=?<>;3@?==?><@???<?>????6><9=?<>;0=?=??;???;?);/=36>>=9>?====??>><>?<>???=>?7::====: /1@D61655M1:276:D10YJACXX:8:1101:1551:1953 NGAGNACATGAGACGGACGTTGTAGAGCATTCGAACTTTGCCAGCAAGCATACTCCACCAGTTTTTTTGAGCTAGAGTGATAATGGTCAGATCGGAAGAG head GAMMA-2.solexa.fastq /2@D61655M1:276:D10YJACXX:8:1101:1456:1955 CACCAATCCAAGCTCCACAACTTGATGTAGTCCGAACAGTTTCATCATACGGTCAAAATCAAATTCAACGTCCATCATTTGACTTAAACGTTCTTCCCTC + C@CFFFFFHHGHHJJJIJJCHIJJIJJHIIHIIIIJJEIHIIIIJGIIJJIIHIHHJJJIJIJJJIJIHHEEFFFEEEE@CDEDDDDDCD<C?@CDCCDD /2@D61655M1:276:D10YJACXX:8:1101:1494:1967 TAAAAATCCTTTAAAACAATATGCTAACTTAGCTAATTTAAAGCCTAGTTTTTGTAAAAAACAACCCGTGTAGCACTGCAATAACTTAAACTTTTGCGTA + ?@BFFFFFDDHGFGGIGGIIEHJGGGIGGIHGIJJIJJJIIJGGGGEHHGGHIJGJJJJIJJIGIGIHHGEFFFFEEEEEEDDDDDDDCCCCDDDCDB>@ /2@D61655M1:276:D10YJACXX:8:1101:1551:1953 GACCATTATCACTCTAGCTCAAAAAAACTGGTGGAGTATGCTTGCTGGCAAAGTTCGAATGCTCTACAACGTCCGTCTCATGTTCTCCAGATCGGAAGAG
