On Tue, Sep 18, 2012 at 10:16 AM, Laurent MANCHON <lmanchon@xxxxxxxxxxxxxx> wrote: > Le 18/09/2012 10:25, Peter Cock a écrit : >> >> Add the /1 and /2 without a space in front of them. >> >> Peter > > ... > > nothing change, i have the same error message. > > head GAMMA-1.solexa.fastq > /1@D61655M1:276:D10YJACXX:8:1101:1456:1955 > ... Apologies - on re-reading my English was ambiguous, although it should have been obvious that isn't a valid FASTQ file as the identifier line must start with '@'. As Jason pointed out, what I meant was your read should start: @D61655M1:276:D10YJACXX:8:1101:1456:1955/1 etc Peter -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html