On Tue, Sep 18, 2012 at 9:18 AM, Laurent MANCHON <lmanchon@xxxxxxxxxxxxxx> wrote: > > something wrong, > after adding /1 and /2 at the end of headers into the respective files i > have the same error: > "Read ...... is present more than once in the data set. Did you load a file > twice in the manifest? Is a read present more than once in your file(s)?" > > head GAMMA-1.solexa.fastq > @D61655M1:276:D10YJACXX:8:1101:1456:1955 /1 > ... Add the /1 and /2 without a space in front of them. Peter -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html