Hi, I have 9582196 mate-paired Solexa seqeunces, 100bp long. The genome I am assembling is somewhere between 700KB and 1.5MB (Mycoplasma). Anyhow, It seems like I rant out of my 500GB local space for the log files. Is that reasonable? I can increase local scratch space, I just want to make sure that I am not doing something wrong. Seems like quite a bit of space to be used for such an assembly. Parameters I used: --project=CCGTCC COMMON_SETTINGS -DI:trt=/localscratch/friedbi/mira_logs --job=denovo,genome,accurate,solexa SOLEXA_SETTINGS -GE:tismin=150:tismax=350 The entire logfile is available here, if someone is interested in more information: http://db.tt/6VxJ8sW Thanks in advance for any help! Iddo -- Iddo Friedberg http://iddo-friedberg.net/contact.html