You can use the program miramem to estimate the needed RAM requirements but I'm not aware of a way to estimate the disk space needed for an assembly. On Mon, Aug 15, 2011 at 9:57 AM, Iddo Friedberg <idoerg@xxxxxxxxx> wrote: > OK. So any rule-of-thumb to calculate the necessary local log space? > > > On Mon, Aug 15, 2011 at 12:46 PM, Evan <evan@xxxxxxxxxxx> wrote: > >> Yes that sounds about right.. >> >> >> On Mon, Aug 15, 2011 at 9:41 AM, Iddo Friedberg <idoerg@xxxxxxxxx> wrote: >> >>> Hi, >>> >>> I have 9582196 mate-paired Solexa seqeunces, 100bp long. The genome I am >>> assembling is somewhere between 700KB and 1.5MB (Mycoplasma). >>> >>> Anyhow, It seems like I rant out of my 500GB local space for the log >>> files. Is that reasonable? I can increase local scratch space, I just want >>> to make sure that I am not doing something wrong. Seems like quite a bit of >>> space to be used for such an assembly. >>> >>> Parameters I used: >>> >>> --project=CCGTCC COMMON_SETTINGS -DI:trt=/localscratch/friedbi/mira_logs >>> --job=denovo,genome,accurate,solexa SOLEXA_SETTINGS >>> -GE:tismin=150:tismax=350 >>> >>> The entire logfile is available here, if someone is interested in more >>> information: http://db.tt/6VxJ8sW >>> >>> Thanks in advance for any help! >>> >>> Iddo >>> >>> >>> -- >>> Iddo Friedberg >>> http://iddo-friedberg.net/contact.html >>> >> >> > > > -- > Iddo Friedberg > http://iddo-friedberg.net/contact.html >