Hello everybody, I'm trying to assembl artificial reads which produced by art which is a next generation sequencing simulator. I do have illumina reads in different coverages. The problem even though I used e.coli genome to produce reads in 10x, 20x, and even 30x coverages I got dozens of contigs and the lenght of the contigs very short. (I meant it because lots of contigs length same with the read lengths (100bp)).After 40x coverage I do get better contig lengths but still the number of contigs so high!!! at 40x coverage I end up with 4000 contigs. I used very basic parameters to run mira. Now I'm reading manuals of it but I almost lost in it. Is there anybody can give me advice that can improve my result? -- Mehmet Göktay, MSc student Department of Molecular Biology and Genetics Izmir Institute of Technology 35430, Urla, Izmir, TURKEY (For the website of Plant Molecular Genetics Laboratory please click here <http://plantmolgen.iyte.edu.tr/>.)