I'm trying to assemble 5 million solexa reads against a drosophila ref. mira -fastq -job=mapping,genome,accurate,solexa -project=DGRP-712 I get an unceremonious killed with no log output apart from what's on the screen. I only have 12 Gb RAM but thought I'd try it after reading the optimistic words from Bastein on 3.2.1.5 regarding memory improvements Am I being too hopeful Done. Loaded 15 reads with 168736537 raw bases. 0 reads have quality accounted for. Postprocessing backbone(s) ... this may take a while. 15 to process 2L_bb 23011544 2LHet_bb 368872 2R_bb 21146708 2RHet_bb 3288761 3L_bb 24543557 3LHet_bb 2555491 3R_bb 27905053 3RHet_bb 2517507 4_bb 1351857 M_bb 19517 Uextra_bb 29004656 U_bb 10049037 X_bb 22422827 XHet_bb 204112 YHet_bb 347038 Localtime: Fri Feb 11 09:29:40 2011 Seeing strain 1: "ReferenceStrain" Generated 1 unique strain ids for 15 reads. Strain "default" has 0 reads. Strain "ReferenceStrain" has 15 reads. Loading data (Solexa) from FASTQ files, Localtime: Fri Feb 11 09:29:40 2011 Counting sequences in FASTQ file: found 5583864 sequences. Localtime: Fri Feb 11 09:32:38 2011 Solexa will load 5583864 reads. Longest Sanger: 0 Longest 454: 0 Longest PacBio: 0 Longest Solexa: 1412 Longest Solid: 0 Longest overall: 1412 Total reads to load: 5583864 -AL:shme is 0, automatically determining optimal value. set -AL:shme 211 -SB:brl is 0, automatically determining optimal value. brl: 3246 -SB:bro is 0, automatically determining optimal value. bro: 1623 Reserving space for reads (this may take a while) tcmalloc: large alloc 1319575552 bytes == 0x2b8d7b000 @ Reserved space for 5687818 reads (including backbone rails). Adding rails to 15 contigs (this may take a while). Killed [rtgood1@imokurok ~]$ ls Cheers Rob Rob Good Ph. +61 3 8344 2347 Mob. 0434058250 Genetics Dept University of Melbourne PARKVILLE, Australia -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html