Hello Bastien, thanks. It worked by re-trying. 256 GB is the amount of memory I'm using. Just 1 CPU core... Another tiny tiny thing: I'm mapping several genomes against a reference and always get just one contig. My supervisor asks how come. What should I reply to this, I mean, is this usual? This is basically good news, but I guess most people here use Newbler for 454 and Velvet for Illumina data and you don't get this using those that easily [actually we've been using Velvet for this and getting ~200 contigs for what MIRA got 1]. Thanks again. Celia ________________________________ From: mira_talk-bounce@xxxxxxxxxxxxx [mira_talk-bounce@xxxxxxxxxxxxx] on behalf of Bastien Chevreux [bach@xxxxxxxxxxxx] Sent: Friday, July 18, 2014 6:11 PM To: mira_talk@xxxxxxxxxxxxx Subject: [mira_talk] Re: (no subject) Yeah, right ... and your mail *was* to MIRA talk. Looks like I confused a couple of things, sorry :-) On July 18, 2014 at 12:24 AM "Mendez Garcia, Celia" <cmendezg@xxxxxxxxxxxx> wrote: Hi, I get this log file when mapping to a reference bacterial genome. What's the problem here? Thanks, Celia