On Donnerstag 18 März 2010 Peter wrote: > On Thu, Mar 18, 2010 at 7:05 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote: > > MIRA will guess right most of the time which format it sees. But you can > > force it to use a given offset with -LR:fqqo > > Does it care about PHRED (min zero) versus the old Solexa scores > (which went down to -5)? Yes, but only when loaded as FASTA quality ... long story. But I won't fix this quirk for FASTQ anymore as Illumina dropped the negative values (fortunately). Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html