OK, I definitely got a better result this time! I passed from 11K to 2640. Now I can do an hybrid assembly with the SAnger reads, I guess. Question: I only have the fasta sequences and the quality files, calculated with CodonAligner(Phred). How do I create a traceinfo file? Do I have to do it manually? Thanks for your help, Alessandro R. Length assessment: ------------------ Number of contigs: 2640 Total consensus: 12755203 Largest contig: 50164 N50 contig size: 8242 N90 contig size: 2225 N95 contig size: 1453 Coverage assessment: -------------------- Max coverage (total): 413 Max coverage Sanger: 0 454: 445 Solexa: 0 Solid: 0 Avg. total coverage (size >= 5000): 10.10 On Wed, Dec 23, 2009 at 12:10 AM, Bastien Chevreux <bach@xxxxxxxxxxxx>wrote: > On Dienstag 22 Dezember 2009 Alessandro Riccombeni wrote: > > I have sff_extract 0.2.6 > > Well, you should definitively try > > http://www.chevreux.org/tmp/mira_3rdparty_10-12-2009.tar.bz2 > > B. > > -- > You have received this mail because you are subscribed to the mira_talk > mailing list. For information on how to subscribe or unsubscribe, please > visit http://www.chevreux.org/mira_mailinglists.html >