Wow, Thanks Bastien, I do not realise that I need this. I will try it and post the result here. Happy weekend! Marcelo 2012/10/12 Bastien Chevreux <bach@xxxxxxxxxxxx> > On Oct 12, 2012, at 23:16 , Marcelo Brandão wrote: > > [...] > "In file ../map3_manifest.dat: line > -SK:mnr=1:pr=70:hss=2 > has keyword '-sk:mnr' which is not recognised." > > > Ah, I see. You forgot the backslashes at the end of the parameter lines. > From the manual: > > Note > > For improved readability, you can distribute parameters across several > lines either by pre-fixing every line with parameter=, like so: > > job=denovo,genome,draft > parameters= 454_SETTINGS -AS:mrl=80:bdq=10 > parameters= SOLEXA_SETTINGS -AS:mrl=30:bdq=15 > > Alternatively you can use a backslash at the end of a parameter line to > indicate that the next line is a continuing line, like so: > > job=denovo,genome,draft > parameters= 454_SETTINGS -AS:mrl=80:bdq=10 *\* > SOLEXA_SETTINGS -AS:mrl=30:bdq=15 > > > Note the backslash in the second version. > > B. > > -- Marcelo Mendes Brandão Academic Visitor at University of Alberta @ Edmonton Website: http://bioinfo.esalq.usp.br AtPIN: http://bioinfo.esalq.usp.br/atpin SKYPE: mmbrand