On Oct 12, 2012, at 23:16 , Marcelo Brandão wrote: > [...] > "In file ../map3_manifest.dat: line > -SK:mnr=1:pr=70:hss=2 > has keyword '-sk:mnr' which is not recognised." Ah, I see. You forgot the backslashes at the end of the parameter lines. From the manual: Note For improved readability, you can distribute parameters across several lines either by pre-fixing every line with parameter=, like so: job=denovo,genome,draft parameters= 454_SETTINGS -AS:mrl=80:bdq=10 parameters= SOLEXA_SETTINGS -AS:mrl=30:bdq=15 Alternatively you can use a backslash at the end of a parameter line to indicate that the next line is a continuing line, like so: job=denovo,genome,draft parameters= 454_SETTINGS -AS:mrl=80:bdq=10 \ SOLEXA_SETTINGS -AS:mrl=30:bdq=15 Note the backslash in the second version. B.