Hi, glad to have mira and the discussion forum here. I'm a new guy to mira, so not sure I did it right: I have 130Mb assembled scaffolds produced by Solexa reads and 100Mb 454 reads. I'm trying to join the 454 reads into the scaffolds by mira. My command line is : mira -GE:not=8 -fasta -project=hybrid -job=denovo,genome,normal,sanger,454 -notraceinfo where I treated all the scaffolds as 'sanger reads'. Strange thing is, I finally got 'unpadded' scaffolds only sized about 25Mb. Does anyone know if I did something wrong or how to handle my data with mira? Thanks a lot! Qi _________________________________________________________________ Messenger10年嘉年华,礼品大奖等你拿! http://10.msn.com.cn