On Wednesday, October 31, 2012, Robert Trygve Good wrote: > > On 30/10/2012, at 6:37 PM, Bastien Chevreux wrote: > > > On Oct 30, 2012, at 4:51 , Robert Trygve Good > > <rtgood@xxxxxxxxxxxxxx<javascript:;>> > wrote: > >> Further to this thread the sam file has a line that contains a message > about being assembled by mira. > >> Tablet will not load this file unless these lines are removed > > > > Which version of tablet? > linux 1.12.08.29 > And what is the problem line? Could you copy and paste it here, and also check with hexdump or similar if the white spaces are tabs. And are you looking at the SAM file directly, or converting it to a sorted and indexed BAM first? The SAM and BAM parsers in Tablet act a little differently. Peter