On 17 Mar 2015, at 18:21 , lenis vasilis <val1@xxxxxxxxxx> wrote: > In that case, If its possible to have intels, how the unpadded file can be ok? A picture being worth a thousand words, have a look at this; http://mira-assembler.sourceforge.net/docs/DefinitiveGuideToMIRA.html#chap_intro::srmc_in_454sxahyb_1stpass.png The pads you see in the CONSENSUS line are the ones you see in the padded FASTA file. And I have to agree with Andrej here: you absolutely want to visualise your data in gap4/gap5/Tablet or whatever. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html