Hi Bastien, I recently attempted an assembly using MIRA 3.9.4. The assembly ended with an error though all the output files seem to be present and intact. The assembly seemed to be running fine for about 360 hours.. Here are the last 100 lines of the output below: Pid: 48519 PPid: 48512 TracerPid: 0 Uid: 503 503 503 503 Gid: 503 503 503 503 Utrace: 0 FDSize: 256 Groups: 503 VmPeak: 75124652 kB VmSize: 75068216 kB VmLck: 0 kB VmHWM: 72407756 kB VmRSS: 68010380 kB VmData: 74965816 kB VmStk: 384 kB VmExe: 5136 kB VmLib: 0 kB VmPTE: 133032 kB VmSwap: 0 kB Threads: 1 SigQ: 0/2066390 SigPnd: 0000000000000000 ShdPnd: 0000000000000000 SigBlk: 0000000000000000 SigIgn: 0000000000000000 SigCgt: 0000000180000000 CapInh: 0000000000000000 CapPrm: 0000000000000000 CapEff: 0000000000000000 CapBnd: ffffffffffffffff Cpus_allowed: ffffffff,ffffffff Cpus_allowed_list: 0-63 Mems_allowed: 00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,00000000,000000ff Mems_allowed_list: 0-7 voluntary_ctxt_switches: 6146365 nonvoluntary_ctxt_switches: 1689789 -------------------------------------------------------------------------------- Information on current assembly object: AS_readpool: 26988832 reads. AS_contigs: 0 contigs. AS_bbcontigs: 0 contigs. Mem used for reads: 184 (184 B) Memory used in assembly structures: Eff. Size Free cap. LostByAlign AS_writtenskimhitsperid: 26988832 103 MiB 0 B 0 B AS_skim_edges: 0 16.8 GiB 16.8 GiB 0 B AS_adsfacts: 243715253 5.6 GiB 1.1 GiB 4 B AS_confirmed_edges: 487430504 11.2 GiB 2.1 GiB 0 B AS_permanent_overlap_bans: 1 24 B 0 B 0 B AS_readhitmiss: 0 24 B 0 B 0 B AS_readhmcovered: 0 24 B 0 B 0 B AS_count_rhm: 0 24 B 0 B 0 B AS_clipleft: 26988832 103 MiB 0 B 0 B AS_clipright: 26988832 103 MiB 0 B 0 B AS_used_ids: 0 26 MiB 26 MiB 0 B AS_multicopies: 26988832 26 MiB 0 B 0 B AS_hasmcoverlaps: 0 26 MiB 26 MiB 0 B AS_maxcoveragereached: 26988832 103 MiB 0 B 0 B AS_coverageperseqtype: 0 24 B 0 B 0 B AS_istroublemaker: 26988832 26 MiB 0 B 0 B AS_isdebris: 26988832 26 MiB 0 B 0 B AS_needalloverlaps: 26988832 26 MiB 0 B 0 B AS_readsforrepeatresolve: 0 40 B 0 B 0 B AS_allrmbsok: 0 24 B 0 B 0 B AS_probablermbsnotok: 0 24 B 0 B 0 B AS_weakrmbsnotok: 0 24 B 0 B 0 B AS_readmaytakeskim: 0 40 B 0 B 0 B AS_skimstaken: 0 40 B 0 B 0 B AS_numskimoverlaps: 0 24 B 0 B 0 B AS_numleftextendskims: 0 24 B 0 B 0 B AS_rightextendskims: 0 24 B 0 B 0 B AS_skimleftextendratio: 0 24 B 0 B 0 B AS_skimrightextendratio: 0 24 B 0 B 0 B AS_usedtmpfiles: 49 2 KiB 0 B 0 B Total: 36638261344 (34.1 GiB) ================================================================================ Dynamic allocs: 68 Align allocs: 68 Fatal error (may be due to problems of the input data or parameters): "Now, this is embarassing: could not rollback checkpoint rename after failed snapshot? Error message is boost::filesystem::rename: No such file or directory: "Dientamoeba_scaffold_assembly/Dientamoeba_scaffold_d_chkpt_tmp", "Dientamoeba_scaffold_assembly/Dientamoeba_scaffold_d_chkpt"" ->Thrown: void Assembly::performSnapshot(uint32 actpass) ->Caught: main Aborting process, probably due to error in the input data or parametrisation. Please check the output log for more information. For help, please write a mail to the mira talk mailing list. Subscribing / unsubscribing to mira talk, see: //www.freelists.org/list/mira_talk CWD: /scratch/work/scaffold.assembly.mira.3.9.48512 Thank you for noticing that this is *NOT* a crash, but a controlled program stop. --------------------------------------------------------------------------------------------------------------------- Any insights into what could have caused this and how I could fix it? Let me know if you require further information. Thanks in advance for your support Bastien. Cheers! Joel