[mira_talk] Re: Nanopore support

  • From: Andreas Leimbach <aleimba@xxxxxxx>
  • To: <mira_talk@xxxxxxxxxxxxx>
  • Date: Mon, 7 Mar 2016 15:55:16 +0100

Hi Adrian,

haven't worked with Nanopore data myself yet, but maybe I can give you a lead and you haven't stumbled across the following tools.

At the moment the best choice for Nanopore data seems to be Canu (https://github.com/marbl/canu, further development of the Celera Assembler) following up with Nanopolish (https://github.com/jts/nanopolish). SPAdes supports Nanopore as well but Canu seems to be superior.

Mira with its greedy and OLC algorithms might actually be a good option for Nanopore reads. Would be interesting to compare assemblies.

Hope this is useful information.

Cheers,
Andreas

--
Andreas Leimbach
Universität Münster
Institut für Hygiene
Mendelstr. 7
D-48149 Münster
Germany

Tel.: +49 (0)551 39 33843
E-Mail: aleimba@xxxxxxx

On 07.03.2016 15:33, Adrian Pelin wrote:

Hello,

I was wondering if there would be support eventually for nanocorrected data
of nanopore reads. These can now achieve error rates below 10%.

I am trying to feed these as pacbiohq, however am getting very fragmented
assemblies.

Adrian


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