[mira_talk] Re: 4 won't run!

  • From: Andrej Benjak <abenjak@xxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Wed, 09 Apr 2014 14:34:20 +0200

Hi Theo,

If the manifest is the same as from your first mail, then the problem might be that you didn't define your PEs properly.

In your manifest, it should be (at least):

readgroup = Someunpairediontorrentreads
*autopairing*
data = /home/Wriddhiman/M_Series_Assembly/Raw_Fastq/M10_8.fastq
technology = iontor



Andrej


On 04/09/2014 02:20 PM, Theo.Allnutt@xxxxxxxx wrote:
Hi,

ok, I think I fixed the file not opening problem, though don't know how. I put 
eveything in the same folder and that seemed to work.
Then I got the dreaded NFS warning, which is wrong because I'm using a 
VirtualBox Linux shared folder, not an NFS.

I fixed that with the suggested switch, but then got the error:

* MIRA found duplicate read names in your data (see log above for more info).  *
*                                                                              *
* This should never, never be!                                                 *
*                                                                              *
* You may have loaded a file more than once in the manifest or                 *
* reads may be present multiple times across your input file(s).               *
* Either way: fix that!

However, I ahve checked and none of the reads have the same name. There are 
paired reads with same name then /1 or /2 appended (by sff_extract). Is this 
the problem Has the required format changed since 3.xx?

Thanks.

-----Original Message-----
From: mira_talk-bounce@xxxxxxxxxxxxx [mailto:mira_talk-bounce@xxxxxxxxxxxxx] On 
Behalf Of Bastien Chevreux
Sent: Wednesday, 9 April 2014 5:49 PM
To: mira_talk@xxxxxxxxxxxxx
Subject: [mira_talk] Re: 4 won't run!

On April 9, 2014 at 9:05 AM Theo.Allnutt@xxxxxxxx wrote:
Every time I run Mira 4, I get a file not found error:
Looking for files named in data ...Data
'/path/is/definitely/correct/paired1.fastq' was not found (neither as
file nor as directory) or led to a read error.
What could be wrong? Its driving me mad, have tried with several
different files.
[...]
data = /path/is/definitely/correct/paired1.fastq
As first approximation, I would still guess there's a typo somewhere (I know, I 
know, you checked).

If you type "ls " and then copy/paste (no typing, really mouse-only copy/paste) the full 
path from the "data=" line of the manifest into your terminal and then hit RETURN, does 
this work?

Should that work we'd have to move on to more esoteric explanations like "is MIRA 
run as another user which might not have the same access permissions as you" and 
similar things.

Hope that helps,
   Bastien

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