[mira_talk] Re: 4 won't run!
- From: Tibor Nagy <black00710@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Wed, 9 Apr 2014 07:32:23 +0000
Maybe a permission problem? (Mira run with other permissions than you)
On Wed, Apr 9, 2014 at 7:05 AM, <Theo.Allnutt@xxxxxxxx> wrote:
>
> Every time I run Mira 4, I get a file not found error:
> Looking for files named in data ...Data
> '/path/is/definitely/correct/paired1.fastq' was not found (neither as file
> nor as directory) or led to a read error.
>
> What could be wrong? Its driving me mad, have tried with several different
> files.
>
> Thanks.
>
>
> Manifest file:
> -----------------------------------
> project = paired1
> job = genome,denovo,accurate
>
> readgroup = Paired1
> autopairing
> data = /path/is/definitely/correct/paired1.fastq
> technology = 454
> ------------------------------------
> My fastq file looks like this (have checked, it has only \n returns):
> -----------------------------------------
> @IOFHIWU01A6LKJ/1
>
> TATTTAGTCATATCTCCGATAAACAACACGTCCGTATCTGAAAAATTAGACGCCGTTTCAGGATAAAAATAATTGCTGCCTACGTTGCGGCCGCCAGTAATGTATGCAACATTGTCAACAATAAACAATTTGTTGTGCATGCGCGAATTCAATCGGTTAAAATCAGCCAAAAATTGTGGGTAATACAATAAGCGTGAACGATTGGCCACAGGATTAAAGACTTTGACTTCTATGTTTGGATGCTGATTTAACAACATCACATCAACAATGTCTGATTTAGTACCATAATCATCCAGCAAAATACGTATCTTTACGCCACGATCGGCCGCATTTTTTAATCACCAATTAAAACCTTGGACGAAAAATCATTACTGTAAATATAG
> +
>
> IIHHHIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIII?????IIIIIIIIIIHHHIIIIII;;;;;IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIII@
> @@@IIIIII>>???IIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHIIIIIHHHIIGGGIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIII???I?=??IIIIIIIIIIIIIIA@
> @=;/;;IIII599;HIIIIIIIIEEE=744700//00>>2BBEEIIIIAAAAIIIIII;;744666FBB00226220008/=EE
> @IOFHIWU01A6LKJ/2
>
> CTGCCTTTGTATGTTACATCCTGGCACATGTTCAAAACAGACATGCACATACCATAATTCTTGCCATCTGAATCATCGTGATATCCGA
> +
> EEEEEDDDC=CAEEEEEEEEEEEEEEEEEE@@@77777-@@CCAACCAACAEEEEEEE@7449CEEC
> @@@AEEEEEE<<<<C@@<<<<
> @IOFHIWU01DCPNE
>
> GCTTTGGAAAGCGGCAAATCGTGGTGTGCAGGTGCGCATACTCATCGATGATAATGGCTTAGATTCGGATTTTTCAGACATTATCACACTAGATTCACACCCAAATATTGAGGTTAAGATTTTTAATCCCTACAAAAACCGCTCTAAAATATTGCGTTATCCAGAAATGGTGTATGACTTCAATCGTATCAACCATCGTATGCACAACAAAATCTTTGTAGTCGATGATATTGCACCTATTATCGGCGGGCGCAATGTCGCAGATGACTATTTTGATAATAATACACATCTAAATTTTACTGATACTGATGTGGTGTTTATCGGCGATGTGGTCAAAGCCGCGACAAAGAGCTTTATGTCTTATTGGCACTATGATCGTTCTATACCCGCGACACTTTTGCCTTCAAAGCGATCAATGAAAAATTAATTTCAAAGAAATAAAGGCAATTTCACAAGAAATCCAAGCCCAGCCTAGTCGTAT
> +
>
> IIHHHHH666HIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII==???IIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIIIIIIIIIII?????IIIHHHIII;;;;;HHIIIII;;;;H>HHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIICCCBHHCCCDAIIIIIIIIIIIIIIIIIIIIIIIIIIHHBBBIIH???HIIIIIIIIIIIIE5555?C555IIIIIIIIIIIIHHHCCCCIIIIIIIIIIIIIIIIIIH???CIIIIIIIIIIID;555?EIIIICCCCHIIIIHHGH7??HHIIIIIIIIIIIIHHHIIFFFFEA9447?6EE@
> @E::=>;;;;;7?CBIIFIIIIIII54444<<++444A<==@<<:<///AAACC?666<;@@@==;<<<EAA???@@A@6554
> :?@@4
> @etc...
>
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