-- Genome assembly is a big problem moreover when you have more and more reads,i just got a genome of 400 Mb finished and I can tell i was monitoring the RAM of the server for many weeks...
I have used the latest ABYSS with 320 millions of reads. As for other solutions: - there is a new release of Velvet that should work with large data amounts - SOAPdenovo yields good results, although the distributed binary does not allow for all options described in the documentation (some of those are simply hard coded).I have tested Mira for Paired-end assembly and with million of reads mira is a beast that consumes a lot of RAM ...and has a bad memory footprint.
Laurent -- Bastien Chevreux a écrit :
On Montag 22 November 2010 Tony Travis wrote:[...] The machine I'm running the job on has 64GiB RAM, so 55GiB was not a problem, until the MIRA memory allocation increased to 96.5GiB RAM andthe server started thrashingOh, and I forgot: for a couple of hundred Euro, buy a large and fast SSD, put swap onto that ... and memory trashing is just a minor nuisance and slowdown, not the big problem it is with hard disks :-)B.