On Oct 31, 2012, at 20:43 , John Nash <john.he.nash@xxxxxxxxx> wrote: > 17-fold coverage for 454 is way too low. Try 40-fold. Agreed, that would be the main reason. Though 703 contigs still seems high. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html