[mira_talk] Re: exiting mira without error message

  • From: Chris Hoefler <hoeflerb@xxxxxxxxx>
  • To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
  • Date: Thu, 15 May 2014 16:48:07 -0500

>
> I wonder why sequencing providers still dare to deliver “non-corrected
> only” projects. I always have “delivery of corrected reads” as conditio
> sine qua non when ordering a PacBio sequencing.
>

It does seem like it should be a routine practice, as long as you can still
get the raw data as well. I sometimes like to do my own tweaks to the
error-correction process.


On Thu, May 15, 2014 at 4:37 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote:

> On 15 May 2014, at 20:53 , Chenling Antelope <chenlingantelope@xxxxxxxxx>
> wrote:
>
> My genome is about 1.4 Mb, and I have 28245 reads with median length
> ~2000. I am really inexperienced with pacbio, so could you clarify what you
> mean by filter? (I am assembling a parasite genome from sequencing results
> from whole organism by pulling out reads with homology with a closely
> related genome and then extracting those reads.)
>
>
> I wonder why sequencing providers still dare to deliver “non-corrected
> only” projects. I always have “delivery of corrected reads” as conditio
> sine qua non when ordering a PacBio sequencing.
>
> B.
>
>
>


-- 
Chris Hoefler, PhD
Postdoctoral Research Associate
Straight Lab
Texas A&M University
2128 TAMU
College Station, TX 77843-2128

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