> > I wonder why sequencing providers still dare to deliver “non-corrected > only” projects. I always have “delivery of corrected reads” as conditio > sine qua non when ordering a PacBio sequencing. > It does seem like it should be a routine practice, as long as you can still get the raw data as well. I sometimes like to do my own tweaks to the error-correction process. On Thu, May 15, 2014 at 4:37 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote: > On 15 May 2014, at 20:53 , Chenling Antelope <chenlingantelope@xxxxxxxxx> > wrote: > > My genome is about 1.4 Mb, and I have 28245 reads with median length > ~2000. I am really inexperienced with pacbio, so could you clarify what you > mean by filter? (I am assembling a parasite genome from sequencing results > from whole organism by pulling out reads with homology with a closely > related genome and then extracting those reads.) > > > I wonder why sequencing providers still dare to deliver “non-corrected > only” projects. I always have “delivery of corrected reads” as conditio > sine qua non when ordering a PacBio sequencing. > > B. > > > -- Chris Hoefler, PhD Postdoctoral Research Associate Straight Lab Texas A&M University 2128 TAMU College Station, TX 77843-2128