On 15 May 2014, at 20:53 , Chenling Antelope <chenlingantelope@xxxxxxxxx> wrote: > My genome is about 1.4 Mb, and I have 28245 reads with median length ~2000. I > am really inexperienced with pacbio, so could you clarify what you mean by > filter? (I am assembling a parasite genome from sequencing results from whole > organism by pulling out reads with homology with a closely related genome and > then extracting those reads.) I wonder why sequencing providers still dare to deliver “non-corrected only” projects. I always have “delivery of corrected reads” as conditio sine qua non when ordering a PacBio sequencing. B.