[mira_talk] Re: SFF_EXTRACT
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Mon, 2 Jun 2014 14:21:24 +0200 (CEST)
If the seq_crumbs does not create FASTA files, you can use the one from the MIRA
package.
BTW, MIRA 4.x does not need TRACEINFO files anymore and works nicely with FASTQ.
B.
> On June 2, 2014 at 1:25 PM Bobby Paul <bobbypaul29@xxxxxxxxx> wrote:
>
> Dear Peter,
>
> I changed the file name and tired with this command
>
> ./sff_extract sample.sff -o sample.fastq
>
> Yes it generating sample.fastq file
>
> I am looking for sequence in multi-fasta format.
>
> Earlier I converted SFF file to sequence, quality and traceinfo files
>separately using sff_extract program. By my mistake I missed that sff_extract.
> I am looking three separate files from my SFF file.
>
> I also used fastq_to_fasta from FASTX-toolkit. It showing some error so I
>thought to use SFF_EXTRACT to get the sequence and quality file.
>
> Expecting your valuable suggestions.
>
> Thanks
> Bobby Paul
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