On June 2, 2014 at 11:59 AM Bobby Paul <bobbypaul29@xxxxxxxxx> wrote: > I used sff_extract from seq_crumbs and version: 0.1.8. > There is no error message but it generated one file named sample (without any >file extension). Its looks like fastq file. The seq_crumbs version of sff_extract is not maintained in the MIRA package, but by Jose Blanca at COMAV. Well, and your file which looks like FASTQ will very probably contain the data from the SFF file, so there's your sequence file :-) B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html