[mira_talk] Re: Presenting PCR data as paired end reads?
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Mon, 28 Jun 2010 19:15:03 +0200
On Montag 28 Juni 2010 Peter wrote:
> [...]
> However, suppose we don't do the Sanger sequencing. What if we have
> just the fact that a pair of PCR primers amplified a region of about
> (say) 1000bp? This could be presented as a paired end read (the
> forward and reverse primer sequences of say 20bp each). Do you think
> it would be useful to try and present this information to MIRA or are
> such "reads" just too small?
I'd say "too small". Though 20bp is around the lowest overlap I'd recommend
using, so you might give it a try. Might work, might not. But it will fail
miserably if the gap you are trying to close is repetitive.
Bastien
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