[mira_talk] MIRA run failing due to megahubs
- From: "Offord, Victoria" <vofford@xxxxxxxxx>
- To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
- Date: Wed, 25 Jun 2014 15:36:14 +0000
Hi,
My MIRA run on a parasite transcriptome keeps ending with:
You have 0.1052271917% of your reads as megahubs.
You have set a maximum allowed ratio of: 0.0000000000
Ending the assembly because the maximum ratio has been reached/surpassed.
Failure, wrapped MIRA process aborted.
Does anyone have any advice on how I can avoid this? I have copied in the
manifest below.
Thank you in advance,
Victoria
project = MergedAssembly
job = est,denovo,accurate
parameters = -DI:trtmira.4574 -NW:cmrnl=no -NW:ctp=no -HS:mnr=yes:nrr=15
454_SETTINGS -AL:egp=no
readgroup = illumina
data = L008_R1_001.fastq L008_R2_001.fastq
technology = solexa
segment_placement = ---> <---
readgroup = 454_schicht
data = schicht.fastq
technology = 454
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