[mira_talk] Re: EST orientation
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Wed, 28 Jul 2010 20:49:21 +0200
On Dienstag 27 Juli 2010 Federico Giorgi wrote:
> While assemblying ESTs (mode: est,accurate), can MIRA merge reads with
> opposite orientation?
Yes.
> In other words, can the final contigs represent
> complemented sequences?
Yes.
> In my job, I have to be sure that all the
> contigs are in the same orientation as the original reads.
Umm, not sure I understand you: you want that MIRA does not use reverse
complemented sequences in the assembly?
If yes: this would be the third time in 2 years someone asked for this. I
could implement a switch to perform this (it's 15 minutes of work).
B.
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