[mira_talk] Re: BAC vector sequece masking for de novo assembly using PacBio C2

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Tue, 10 Dec 2013 15:07:25 +0100 (CET)

> On December 10, 2013 at 2:36 PM Juan Pascual Anaya <jpascualanaya@xxxxxxxxx>
> wrote:
> I am sort of scared to increase the -SK:mmhr... although I have seen in other
> posts that it can be set
> to 100 if one wants the repeats also assembled... like in my case (so I should
> use then -HS:mnr=off).

Yes, simply turn off -HS:mnr and increase -SK:mmhr to 100. No reason to be
scafred, your computer won't undergo a nuclear meltdown or such. Though your
disk might, but you'd find out soon enough if the assembly takes ages.

B.

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