[LRflex] Re: I made it on the cover
- From: "Fred Hess / Phenix Visuals NL" <fredhess@xxxxxxxxxxxxxxxxx>
- To: <leicareflex@xxxxxxxxxxxxx>
- Date: Thu, 13 Sep 2007 16:23:17 +0200
Hello Jonathan,
Thank you for your reply.
I know the techniques you used to create this picture, the result is
very beautifull!
I also use a DIC-set on my Orthoplan, a beautifull way to
create stunning images!
To my opinion and experience the US$500,-- (or are they Can. $$ ?)
is a good investment to attach the Leica R/DMR to a microscope.
As I already mentioned it works great, especialy in combination with the
Imacon Flexcolor software.
Kind regards Fred Hess
----- Original Message -----
From: <jlee@xxxxxxxxxx>
To: <leicareflex@xxxxxxxxxxxxx>
Sent: Thursday, September 13, 2007 2:48 PM
Subject: [LRflex] Re: I made it on the cover
> Fred,
>
> The image is all epi-fluorescence, illuminated by a UV and visible light
> source. The white of the image comes fome a dye that fluoresces red
> (phallodin) and the blue from a Hoescht dye. Actually the best part of
> the image is the "post processing". What happens is that the camera takes
> about 50 individual pictures at different up and down focal planes in the
> picture. A computer algorithm (called deconvolution) then "subtracts" out
> the light scatter and out of foucs bits and then merges all 50 images into
> one. The image becomes quite sharp but it takes about 1 hour of time on a
> Mac G5 to do this. Because the camera is monochrome (they are much more
> sensitive and have less noise than colour ones), the different colours are
> collected at different times with filters in front of the camera. This
> allows us to "pseudocolour" the image verys simply.
>
> We also collect data with DIC (differential interference contrast) for
> "white light" work but this isn't used.
>
> In microscopy, Leica competes with Zeiss, Nikon and Olympus and a lot of
> things hold true in both microscopy and photography: IMO Leica microscopes
> have the best ergonomics and to my eyes their objectives are visibly
> better than Nikon, Zeiss and Olympus. Of course they are more expensive
> than the competition by 30% and customer service is so-so. Leica software
> lags behing the competition, where I think that Olympus is best.
>
> I've often thought about putting my R9/DMR on my Leica scope but I just
> can't bring myself to shell out the $500 for the adapater. Digital SLRs
> are just not sensitive enough for fluorsenct use. In the pre-digital age,
> I did put my M6 onto an old Leitz light microscope (no adapter required)
> and it worked perfectly for sections and the like. The meter was quite
> handy for this.
>
> Jonathan,
> >
> > Very nice picture. Congratulations for the publication on the
> > magazinecover.
> > What kind of technique did you use?
> > Fluorescence? DV of oblique reflective fluorescence?
> >
> > I am mainly a wildlife photographer
> > but I also have some Leitz/Leica microscopes (Orthoplan, Dialux and
> > HM-Lux).
> > I am a keen microscopist, taking pictures of marine zooplankton.
> >
> > I use the Leica R8/DM-R-combination on these scopes. Great combination
> > for this kind of work. And really cheap, indeed ;-))) (Compared to your
> > set-up).
> >
> > Kind regards
> >
> > Fred Hess.
> >
> >
> >
> >
> > ----- Original Message -----
> > From: <jlee@xxxxxxxxxx>
> > To: <leicareflex@xxxxxxxxxxxxx>
> > Sent: Thursday, September 13, 2007 5:16 AM
> > Subject: [LRflex] I made it on the cover
> >
> >
> >> First time my work has been on the cover of something:
> >>
> >> http://mcr.aacrjournals.org/current.shtml
> >>
> >> We'll it's not reflex photography but it was taken through a Leica DM1L
> >> microscope with a Leica 63X APO objective. If you think that Leica
> >> photo
> >> optics are expensive, this tiny piece of glass (it's about the size of
a
> >> thumb) cost almost $10K. Microscope is $50K and the monochrome only
> >> camera
> >> cost $20K. An R9/DMR is a bargain in comparison.
> >>
> >> The picture has been pseudocoloured in that what appears as white was
> >> actually red. Blue is really blue. The actual size of the image is
> >> about
> >> 0.25mm x 0.5mm.
> >>
> >> Jonathan
> >>
> >>
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> >
> >
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