On 03/28/2010 05:30 PM, Bastien Chevreux wrote:
I just gave another try...I mapped again reads vs the backbones, this time including the previously assembled and missing contig.This is ... interesting. For whatever reason, MIRA thinks it is a good idea to cut back these reads. Why it thinks that I have no idea and needs to be investigated.
Now mira pulls out other 2356 reads, but they do not cover the contig for the whole length, and the sanger reads are still not used....
Is this version of mira too stringent? -- Dr. Stefano Ghignone Istituto per la Protezione delle Piante, Sez. Torino - CNR c/o Dpt. Plant Biology, University of Turin V.le P.A. Mattioli, 25 I-10125 Turin Italy Phone: +39 011 6502927 ext. 48 Fax: +39 011 6705962 e-mail: stefano.ghignone@xxxxxxxx MSN: ste.ghi@xxxxxxxxxxx http://www.bioveg.unito.it http://www.sppadbase.com http://www.dinamycode.it/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html