Yes. I recently went through a situation w/ an slightly older gal (not mine, I was on the stud dog end), who it turns out did not ovulate. For anyone who does timing, you'll know that at 2+ the LH surge begins and although most check again, you figure breed in 3-4 days. (It is actually the LH surge and NOT progesterone that gets the stud dog interested). Generally you need 5 for ovulation, figure 2-3 days for the eggs to ripen to where they can be fertilized, but unless you've been testing right along, you don't know how quickly a gal has come up or just when she hit a 5, so doing a natural breeding you would breed on a 5 (and you may be a day early), and then breed 2 days later (unless you are using chilled, you'd wait 2 days or for frozen until the very high teens even 20). Following ovulation, progesterone spikes, then levels off. Well, anyway, this gal got to 4.3, then back down and stayed in the 2ish range, finally dropping to below 1. You never would have suspected from the behavior of both the bitch (receptive) or the dog (interested), and without continued testing we never would have known she didn't ovulate. (It got me to thinking how many times bitches might be bred, things may appear normal, but there are no puppies and either the stud dog gets blamed and/or the bitch is fed handfuls of antibiotics the next season because who-knows-what is blamed.) OK next bitch. Completed testing, everything worked like clockwork BUT several days later she was still looking for her man <G>. I ran her in for a progesterone test to verify she had ovulated, and she had, (she just liked her man <G>). There is another reason to do progesterone timing w/ a bitch with a history of unsuccessful pregnancy/reabsorbing. If progesterone drops below 3.5 during pregnancy, the bitch will not maintain her pregnancy and will reabsorb, or if she's far enough along, will abort. Ever have a bitch who looks pregnant and then all of a sudden she doesn't and you have no puppies? Now you get into the whole "what's right" thing about intervening. If you think this is due to advancing age and NOT a genetic/familial issue, you can monitor/supplement progesterone during the last half of the pregnancy. Gosh I wish I didn't know this stuff! Remember when you were a kid and one neighbor's dog courted another neighbor's dog and 9 weeks later you were over their house looking at furry babies?????? My mother, (bless her!), known for her pithy comments, usually has a few choice words when I am in the middle of doggy reproductive nonsense. "Dogs used to do it in the street dear, all by themselves without help. I don't know why you and your friends make such an issue of it!" I remember speaking to her when the second girl mentioned above was screaming for her man, days after she shouldn't have been receptive and changing my plans for that day to include a trip to the vet for a progesterone test. "Just let them 'do it' hon, Mom said. Why do you need to have that vet take more blood?" We went to the vet, lol. Kathy, member GSDCA, DVGSDC Celebrating generations of Dual Titled TC'd Champions visit www.geocities.com/pinehillgsds In a message dated 8/16/2008 8:00:58 P.M. Eastern Daylight Time, texasgsds@xxxxxxxxx writes: Hello, Do any of you guys do progesterone testing after having bred a bitch. If so, when do you test? Thanks. Diane **************Looking for a car that's sporty, fun and fits in your budget? Read reviews on AOL Autos. (http://autos.aol.com/cars-Volkswagen-Jetta-2009/expert-review?ncid=aolaut00030000000007 ) ============================================================================ POST is Copyrighted 2007. All material remains the property of the original author and of GSD Communication, Inc. NO REPRODUCTIONS or FORWARDS of any kind are permitted without prior permission of the original author AND of the Showgsd-l Management. ALL RIGHTS RESERVED. ALL PERSONS ARE ON NOTICE THAT THE FORWARDING, REPRODUCTION OR USE IN ANY MANNER OF ANY MATERIAL WHICH APPEARS ON SHOWGSD-L WITHOUT THE EXPRESS PERMISSION OF ALL PARTIES TO THE POST AND THE LIST MANAGEMENT IS EXPRESSLY FORBIDDEN, AND IS A VIOLATION OF LAW. VIOLATORS OF THIS PROHIBITION WILL BE PROSECUTED. For assistance, please contact the List Management at admin@xxxxxxxxxxxx VISIT OUR WEBSITE - www.showgsd.org ============================================================================