2012/4/13 Chauhan, Archana <achauha1@xxxxxxx> > > Attached please find the log file. I do not understand (i)The NFS system. How do I modify the assembly command to redirect the tmp directory somewhere else (ii)What is SSD (iii)Why is mira also using Sanger files/data when I am not assembling sander data? Thank you. Hmm, just to answer these questions, (i) Have a look at the error message at the end of the log; there is clearly stated: 1) RECOMMENDED! Use -DI:trt to redirect the tmp directory somewhere else on a local disk or even SSD. (ii) Solid State Drive, http://lmgtfy.com/?q=SSD (iii) What makes you think mira will use sanger data? Sven