Hi every body, I am new to mira and de-novo assembly, but I know from earlier project, that there is always a discussion as to how the insert size is defined. Waht does mira calls insert size? Is it the complete length of the two reads plus the distance between them or is it only for that distance? Thanks in advance and I am sorry if it's a trivial question, but I could't find anything in the manual or the lists. cu, Assa -- Assa Yeroslaviz Max Planck Institute for Biology of Ageing / Max-Planck-Institut für Biologie des Alterns Application service, Bioinformatics group / Bioinformatische Servicegruppe office: ZMMK, Robert-Koch-Str. 21 D-50931 Cologne Postal address: Postfach 41 06 23, D-50866 Köln / Cologne +49 (0)221 47889795 Assa.Yeroslaviz@xxxxxxxxxx www.age.mpg.de