[mira_talk] Very long transcripts
- From: Jackie Lighten <jackie.lighten@xxxxxx>
- To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
- Date: Tue, 30 Oct 2012 10:35:54 -0300
Hi,
I wonder if someone can offer some insight.
I have performed an accurate de novo assembly with poly-a/t trimming.
I get all reads assembled, and no singlets, into around 66k contigs. Around
27k of these are large contigs, with the largest being ~25k bases long. This
does not make much sense to me as I constructed a 3' target cDNA library
(454 FLX). I can envisage multiple open reading frames may create longer
transcripts but 25k seems dodgy to me.
Any thoughts?
Best,
Jack
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