On Montag 23 November 2009 Yi Zheng wrote: > The log info is too long so I post it on my blog : > http://kentnf.blogspot.com/2009/11/to-bastien-mira-log.html Ah, I suspect I know where the reads got cut: in the chimera trackdown part. When no clipping is applied, sequencing errors towards (but not quite at) the ends of reads may make it behave like a possible chimera, which is the reason why MIRA cuts them down. You can see this in the log in the lines starting with "Possible chimera". Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html