[mira_talk] Re: Question about turn off end clip.

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Mon, 23 Nov 2009 23:24:17 +0100

On Montag 23 November 2009 Yi Zheng wrote:
> The log info is too long so I post it on my blog :
> http://kentnf.blogspot.com/2009/11/to-bastien-mira-log.html

Ah, I suspect I know where the reads got cut: in the chimera trackdown part.

When no clipping is applied, sequencing errors towards (but not quite at) the 
ends of reads may make it behave like a possible chimera, which is the reason 
why MIRA cuts them down.

You can see this in the log in the lines starting with "Possible chimera".

Regards,
  Bastien

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