[mira_talk] Re: Question about turn off end clip.
- From: "Yi Zheng" <yz357@xxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Mon, 23 Nov 2009 17:35:46 -0500 (EST)
Thank you! I find it : Possible chimera : FTSW1LU02D8I07 [0,115[ cut back
to [60,115[. That why my example was clipped 60bp at left end.
How to turn this function off?
> On Montag 23 November 2009 Yi Zheng wrote:
>> The log info is too long so I post it on my blog :
>> http://kentnf.blogspot.com/2009/11/to-bastien-mira-log.html
>
> Ah, I suspect I know where the reads got cut: in the chimera trackdown
> part.
>
> When no clipping is applied, sequencing errors towards (but not quite at)
> the
> ends of reads may make it behave like a possible chimera, which is the
> reason
> why MIRA cuts them down.
>
> You can see this in the log in the lines starting with "Possible chimera".
>
> Regards,
> Bastien
>
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