[mira_talk] Re: Problem with convert_project

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Sat, 27 Oct 2012 03:17:54 +0200

On Oct 26, 2012, at 21:14 , Steven Sijmons <steven.sijmons@xxxxxxxxx> wrote:
> […]
> When I then compared the number of reads in the fastq file I created with 
> convert_project, I noticed that these contained more reads than the number of 
> lines present in the debris_list. Debris_lists contained 232 628, 2 288 887 
> and 42 826 127 reads, while the convert_project outputs contained 437 106, 2 
> 392 084 and 43 632 082 reads respectively. Could these 'extra' reads be 
> causing this? And where are these reads coming from if they aren't in the 
> debris_list? Hoping that someone can give some possible explanations for 
> this, because I really don't understand what's happening here.

Strange. convert_project should really only extract the reads it is told to 
fetch.

Would you be able to provide the debris list and the original fastq for me to 
have a look?

B.
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