[mira_talk] Re: PacBio for scaffolding?
- From: Brian Forde <bforde@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 19 Jul 2011 09:15:14 +0100
Did PacBio not recently announce that they had increased read lengths to an
average of 2.9kb and with consensus circular sequencing increased accuracy
to 99.998%. I suppose the question then is whether consensus circular
sequencing significantly increases the cost of a project or not.
Brian
On Mon, Jul 18, 2011 at 11:54 PM, Bastien Chevreux <bach@xxxxxxxxxxxx>wrote:
> On Jul 18, 2011, at 23:25 , Robin Kramer wrote:
> > I doubt that pacbio is going to bring much to the assembly world until
> that accuracy improves. The problem is the reads will still need to be
> "error corrected" by competing sequencing technologies(similar to the
> problem that 454 reads have),
>
> Math is simple: if PB gets reads >= 1500 at >=95 %, 454 unpaired reads are
> in danger of extinction.
>
> I don't think that this is out of reach.
>
> Bacteria and lower eukaryotes would then have a mix of PB CCS, 454 PE and
> Solexa. Or perhaps even PB CCS, PB strobed abd Solexa ... but for that the
> single read accuracy of Solexa should go >= 90%.
>
> B.
>
>
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